A deeper understanding of intestinal organoid metabolism revealed by combining fluorescence lifetime imaging microscopy (FLIM) and extracellular flux analyses.

Okkelman, Irina A; Neto, Nuno; Papkovsky, Dmitri B; Monaghan, Michael G; Dmitriev, Ruslan I

Okkelman, Irina A; Neto, Nuno; Papkovsky, Dmitri B; Monaghan, Michael G; Dmitriev, Ruslan I.

Afiliación

Okkelman IA; Laboratory of Biophysics and Bioanalysis, ABCRF, University College Cork, Cavanagh Pharmacy Building, College Road, Cork, T12 K8AF, Ireland.
Neto N; Department of Mechanical and Manufacturing Engineering, Trinity College Dublin, Ireland; Trinity Centre for Biomedical Engineering, Trinity College Dublin, Ireland.
Papkovsky DB; Laboratory of Biophysics and Bioanalysis, ABCRF, University College Cork, Cavanagh Pharmacy Building, College Road, Cork, T12 K8AF, Ireland.
Monaghan MG; Department of Mechanical and Manufacturing Engineering, Trinity College Dublin, Ireland; Trinity Centre for Biomedical Engineering, Trinity College Dublin, Ireland; Advanced Materials and BioEngineering Research (AMBER) Centre at Trinity College Dublin and Royal College of Surgeons in Ireland, Dubli
Dmitriev RI; Laboratory of Biophysics and Bioanalysis, ABCRF, University College Cork, Cavanagh Pharmacy Building, College Road, Cork, T12 K8AF, Ireland; Institute for Regenerative Medicine, I.M. Sechenov First Moscow State University, Moscow, Russian Federation. Electronic address: r.dmitriev@ucc.ie.

Redox Biol ; 30: 101420, 2020 02.

Article en En | MEDLINE | ID: mdl-31935648

Asunto(s)

Proteínas Fluorescentes Verdes/metabolismo; Mucosa Intestinal/citología; Organoides/citología; Receptores Acoplados a Proteínas G/genética; Animales; Comunicación Celular; Glucosa/farmacología; Proteínas Fluorescentes Verdes/genética; Mucosa Intestinal/efectos de los fármacos; Mucosa Intestinal/metabolismo; Análisis de Flujos Metabólicos; Ratones; Microscopía Fluorescente; Técnicas de Cultivo de Órganos; Organoides/efectos de los fármacos; Organoides/metabolismo; Fosforilación Oxidativa; Receptores Acoplados a Proteínas G/metabolismo; Proteínas Recombinantes/metabolismo; Nicho de Células Madre/efectos de los fármacos; Células Madre/citología; Células Madre/efectos de los fármacos; Células Madre/metabolismo

Palabras clave

Hypoxia imaging; Intestinal organoids; NAD(P)H; Optical metabolic imaging; Oxygen; Stem cells

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Organoides / Receptores Acoplados a Proteínas G / Proteínas Fluorescentes Verdes / Mucosa Intestinal Límite: Animals Idioma: En Revista: Redox Biol Año: 2020 Tipo del documento: Article País de afiliación: Irlanda

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