Long-term in vitro recording of cardiac action potentials on microelectrode arrays for chronic cardiotoxicity assessment.
Arch Toxicol
; 97(2): 509-522, 2023 02.
Article
en En
| MEDLINE
| ID: mdl-36607357
ABSTRACT
The reliable identification of chronic cardiotoxic effects in in vitro screenings is fundamental for filtering out toxic molecular entities before in vivo animal experimentation and clinical trials. Present techniques such as patch-clamp, voltage indicators, and standard microelectrode arrays do not offer at the same time high sensitivity for measuring transmembrane ion currents and low-invasiveness for monitoring cells over long time. Here, we show that optoporation applied to microelectrode arrays enables measuring action potentials from human-derived cardiac syncytia for more than 1 continuous month and provides reliable data on chronic cardiotoxic effects caused by known compounds such as pentamidine. The technique has high potential for detecting chronic cardiotoxicity in the early phases of drug development.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Miocitos Cardíacos
/
Cardiotoxicidad
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Arch Toxicol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Italia