Utilidad clínica de multitest en la evaluación de inmunidad celular / Usefulness of multitest in the assessment of cell immunity

RESUMO

The aim of this work was to assess cellular immunity using the Multitest CMI and relate its results with lymphocyte counts and lymphocyte subpopulations determined using monoclonal antibodies against CD4 and CD8 and fluorescence microscopy. We studied 51 patients (31 males), 20 infected with HIV, 18 with recurring infections, 5 with cancer, 2 with tuberculosis and 6 with miscellaneous diagnoses. According to Multitest results, patients were classified as normal, hypoergic or anergic. Twenty five percent of patients were normal, 65 percent hypoergic and 10 percent anergic. Eighty percent of anergic patients were infected with HIV. No differences in total lymphocyte count were observed between the 3 groups. CD4 lymphocyte count was lower in anergic patients when compared with the other groups. All patients with CD4 counts below 200 cells/mmü were anergic. It is cincluded that Multitest CMI is useful for the assessment of cellular immunity and complements the determination of lymphocyte subpopulations