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Monoclonal antibodies against the human interleukin-11 receptor alpha-chain (IL-11Ralpha) and their use in studies of human mononuclear cells.
Blanc, C; Vusio, P; Schleinkofer, K; Boisteau, O; Pflanz, S; Minvielle, S; Grötzinger, J; Müller-Newen, G; Heinrich, P C; Jacques, Y; Montero-Julian, F A.
Afiliação
  • Blanc C; Immunotech: A Beckman-Coulter Company, Marseille, France.
J Immunol Methods ; 241(1-2): 43-59, 2000 Jul 31.
Article em En | MEDLINE | ID: mdl-10915848
A panel of 14 hybridoma cell lines secreting monoclonal antibodies against the human interleukin-11 receptor alpha chain (hIL-11Ralpha) was obtained using two different approaches. Two antibodies were raised against peptides of the N- and C-terminal sequences, respectively, of the extracellular part of the hIL-11Ralpha. Another group of 12 antibodies was generated against a hybrid protein consisting of the extracellular part of the hIL-11Ralpha fused to mature full-length human IL-2. All these antibodies recognized native hIL-11Ralpha and most also recognized the denatured receptor on immunoblots after SDS-PAGE. Four different epitopes were identified on the extracellular part of the hIL-11Ralpha. One epitope, defined by the E27 antibody, is located at the N-terminus and the other three epitopes are clustered in the membrane-proximal, C-terminal region. The antibodies defining epitopes I and II recognized membrane-bound hIL-11Ralpha expressed in gp130/hIL-11Ralpha-co-transfected Ba/F3 cells. The E27 antibody cross-reacted with murine IL-11Ralpha, in agreement with the fact that the N-terminal region is highly conserved between species. The other 13 antibodies all recognized a region between amino acids 319 and 363, which is the membrane-proximal part of the hIL-11Ralpha. This region, which is less conserved between mouse and human, is shown here to be an immunodominant region. Anti-IL-11Ralpha monoclonal antibodies, which have not been described previously enabled us to explore the expression and tissue distribution of IL-11Ralpha on human peripheral blood mononuclear cells and cell lines. The antibodies provide powerful tools for the study of the regulation and function of the receptor.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Receptores de Interleucina / Citometria de Fluxo / Anticorpos Monoclonais Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: J Immunol Methods Ano de publicação: 2000 Tipo de documento: Article País de afiliação: França
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Receptores de Interleucina / Citometria de Fluxo / Anticorpos Monoclonais Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: J Immunol Methods Ano de publicação: 2000 Tipo de documento: Article País de afiliação: França