Isolation of a new laccase isoform from the white-rot fungi Pycnoporus cinnabarinus strain ss3.
Can J Microbiol
; 46(8): 759-63, 2000 Aug.
Article
em En
| MEDLINE
| ID: mdl-10941525
ABSTRACT
Two extracellular laccase isoforms (Lac I and Lac II) produced by the white-rot fungus Pycnoporus cinnabarinus from the monokaryotic strain ss3 were purified from ferulic-acid-induced liquid culture medium using ammonium sulphate precipitation, followed by anion-exchange chromatography on a Mono Q column. Strain ss3 is the first generation of the parental strain P. cinnabarinus I-937. The new isolated isoform, Lac II, consists of an 86,000 molecular weight protein as determined by SDS polyacrylamide gel electrophoresis. The N-terminal amino acid sequences of both isoforms were determined, and compared to known laccase protein sequences of other organisms.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Basidiomycota
Idioma:
En
Revista:
Can J Microbiol
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
França