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nNOS and eNOS modulate cGMP formation and vascular response in contracting fast-twitch skeletal muscle.
Lau, K S; Grange, R W; Isotani, E; Sarelius, I H; Kamm, K E; Huang, P L; Stull, J T.
Afiliação
  • Lau KS; Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75235-9040, USA. klau@mednet.swmed.edu
Physiol Genomics ; 2(1): 21-7, 2000 Jan 24.
Article em En | MEDLINE | ID: mdl-11015578
Nitric oxide (NO) from Ca(2+)-dependent neuronal nitric oxide synthase (nNOS) in skeletal muscle fibers may modulate vascular tone by a cGMP-dependent pathway similar to NO derived from NOS in endothelial cells (eNOS). In isolated fast-twitch extensor digitorum longus (EDL) muscles from control mice, cGMP formation increased approximately 166% with electrical stimulation (30 Hz, 15 s). cGMP levels were not altered in slow-twitch soleus muscles. The NOS inhibitor N(omega)-nitro-l-arginine abolished the contraction-induced increase in cGMP content in EDL muscles, and the NO donor sodium nitroprusside (SNP) increased cGMP content approximately 167% in noncontracting EDL muscles. SNP treatment but not electrical stimulation increased cGMP formation in muscles from nNOS(-/-) mice. cGMP formation in control and stimulated EDL muscles from eNOS(-/-) mice was less than that obtained with similarly treated muscles from control mice. Arteriolar relaxation in contracting fast-twitch mouse cremaster muscle was attenuated in muscles from mice lacking either nNOS or eNOS. These findings suggest that increases in cGMP and NO-dependent vascular relaxation in contracting fast-twitch skeletal muscle may require both nNOS and eNOS.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: GMP Cíclico / Músculo Esquelético / Óxido Nítrico Sintase / Contração Muscular Limite: Animals Idioma: En Revista: Physiol Genomics Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2000 Tipo de documento: Article País de afiliação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: GMP Cíclico / Músculo Esquelético / Óxido Nítrico Sintase / Contração Muscular Limite: Animals Idioma: En Revista: Physiol Genomics Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2000 Tipo de documento: Article País de afiliação: Estados Unidos