Interaction of antimalarial drug quinacrine with nucleic acids of variable sequence studied by spectroscopic methods.
J Biomol Struct Dyn
; 18(3): 371-83, 2000 Dec.
Article
em En
| MEDLINE
| ID: mdl-11149514
ABSTRACT
The interaction of antimalarial drug quinacrine (QA) with polynucleotides is studied by UV-visible absorption, fluorescence and surface-enhanced Raman spectroscopy (SERS). The polynucleotides employed for such a study were calf thymus DNA, poly(A).poly(T), poly(A).poly(U), poly(C).poly(G) and poly(dG-dC).poly(dG-dC). Absorption and fluorescence spectra of QA complexes indicate that an interaction with the biomolecule is taking place, although different interaction mechanisms are probable depending on the sequence. The SERS spectra also reflect spectral changes which depend on the polymer sequence and that can be correlated to those observed by fluorescence, with the advantage of the detailed structural information provided by this vibrational technique. QA interacts with polynucleotides through its diprotonated form and by ring stacking. The strength of such interaction is extremely sequence dependent, thus suggesting different interaction mechanisms in each case. The SERS technique allows the simultaneous study of those polynucleotide moieties that are directly involved in the interaction thanks to the short-range character of the SERS spectroscopy. The interaction of QA with the above nucleic acids lead to a different change in the chain stability and flexibility which is further related to the different denaturation tendency of the polymer in the presence of the metal surface.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quinacrina
/
Ácidos Nucleicos
/
Antimaláricos
Limite:
Animals
Idioma:
En
Revista:
J Biomol Struct Dyn
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Espanha