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Indomethacin increases 15-PGDH mRNA expression in HL60 cells differentiated by PMA.
Frenkian, M; Pidoux, E; Baudoin, C; Segond, N; Jullienne, A.
Afiliação
  • Frenkian M; INSERM Unit 349, Centre Viggo Petersen, Hopital Lariboisière, 2. Rue Ambroise Paré, Paris Cedex 10, 75475, France.
Article em En | MEDLINE | ID: mdl-11237475
We previously reported an induction of 15-hydroxyprostaglandin dehydrogenase type I mRNA (15-PGDH) expression accompanied by a decrease in prostaglandin E2(PGE2) levels during cord blood monocytes differentiation into preosteoclastic cells by 1,25 dihydroxyvitamin D3 (1,25 (OH)2D3). These results suggested a role of prostaglandin (PG) enzymes in adhesion and/or differentiation of monocytes. In the present work, we studied modulation of gene expression of PG metabolism enzymes mRNAs in HL60 cells differentiated by phorbol myristate acetate (PMA) into the monocyte/macrophage lineage. We showed that adhesion of HL60 induced by PMA causes an increase of cyclooxygenase 2 (COX 2) and 15-PGDH mRNAs. When adding indomethacin, a non steroidal antiinflammatory drug known to inhibit COX activity, the cells remained attached and expressed large amounts of 15-PGDH mRNA while COX 2 mRNA expression remained unchanged. Indomethacin, in association with PMA can consequently exert a dual control on key enzymes of PGE2 metabolism without modifying adhesion of the cells.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidroxiprostaglandina Desidrogenases / Anti-Inflamatórios não Esteroides / Indometacina Limite: Humans Idioma: En Revista: Prostaglandins Leukot Essent Fatty Acids Assunto da revista: ENDOCRINOLOGIA Ano de publicação: 2001 Tipo de documento: Article País de afiliação: França
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidroxiprostaglandina Desidrogenases / Anti-Inflamatórios não Esteroides / Indometacina Limite: Humans Idioma: En Revista: Prostaglandins Leukot Essent Fatty Acids Assunto da revista: ENDOCRINOLOGIA Ano de publicação: 2001 Tipo de documento: Article País de afiliação: França