Molecular mechanisms of the biological clock in cultured fibroblasts.
Science
; 292(5515): 278-81, 2001 Apr 13.
Article
em En
| MEDLINE
| ID: mdl-11303101
ABSTRACT
In mammals, the central circadian pacemaker resides in the hypothalamic suprachiasmatic nucleus (SCN), but circadian oscillators also exist in peripheral tissues. Here, using wild-type and cryptochrome (mCry)-deficient cell lines derived from mCry mutant mice, we show that the peripheral oscillator in cultured fibroblasts is identical to the oscillator in the SCN in (i) temporal expression profiles of all known clock genes, (ii) the phase of the various mRNA rhythms (i.e., antiphase oscillation of Bmal1 and mPer genes), (iii) the delay between maximum mRNA levels and appearance of nuclear mPER1 and mPER2 protein, (iv) the inability to produce oscillations in the absence of functional mCry genes, and (v) the control of period length by mCRY proteins.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Relógios Biológicos
/
Regulação da Expressão Gênica
/
Ritmo Circadiano
/
Células Fotorreceptoras de Invertebrados
/
Proteínas de Drosophila
/
Proteínas de Ligação a DNA
/
Proteínas do Olho
/
Fibroblastos
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Japão