Synthesis of novel internal standards for the quantitative determination of plasma ceramide trihexoside in Fabry disease by tandem mass spectrometry.
FEBS Lett
; 515(1-3): 171-6, 2002 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-11943216
ABSTRACT
The concentration of globotriaosylceramide (ceramide trihexoside (CTH)) in the plasma of patients with Fabry disease has been determined quantitatively by tandem mass spectrometry (MS) using novel internal standards, [D4]C-16 CTH and C-17 CTH, which were synthesised enzymically from lyso-CTH using the reverse reaction of sphingolipid ceramide N-deacylase. C-17 CTH was also synthesised chemically from lyso-CTH. This strategy has also been used to prepare standards for the quantitative determination by MS of other glycosphingolipids.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicoesfingolipídeos
/
Doença de Fabry
Limite:
Humans
/
Male
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Reino Unido