Platelet-activating factor enhances the expression of vascular endothelial growth factor in normal human astrocytes.
Brain Res
; 944(1-2): 65-72, 2002 Jul 19.
Article
em En
| MEDLINE
| ID: mdl-12106666
ABSTRACT
Vascular endothelial growth factor (VEGF) is a potent and specific mitogen for vascular endothelial cells. To examine whether platelet-activating factor (PAF) induces the expression of VEGF in human astrocytes, we stimulated cultured normal astrocytes with PAF and performed semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) or real-time quantitative PCR for VEGF mRNA and enzyme-linked immunosorbent assay for VEGF protein. PAF increased the expression of VEGF in astrocytes in time- and dose-dependent manners. After 24-h stimulation, 10 nM PAF increased the levels of VEGF protein in astrocyte-conditioned medium by 1.3-fold. When the cells were subjected to hypoxia, the PAF-induced production of VEGF was enhanced by 6.7-fold as compared to the unstimulated cells incubated under normoxia. Dexamethasone was found to inhibit the enhanced VEGF production in response to the stimulation with PAF under hypoxia. We conclude that PAF induces VEGF gene expression in human astrocytes, and the PAF-induced increase in the expression of VEGF may modulate nervous tissue injury due to hypoxia.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Fator de Ativação de Plaquetas
/
Regulação para Cima
/
Hipóxia Encefálica
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Astrócitos
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Fatores de Crescimento Endotelial
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Linfocinas
/
Degeneração Neural
Limite:
Humans
Idioma:
En
Revista:
Brain Res
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Japão