Delayed hypothermia prevents decreases in N-acetylaspartate and reduced glutathione in the cerebral cortex of the neonatal pig following transient hypoxia-ischaemia.
Neurochem Res
; 27(12): 1599-604, 2002 Dec.
Article
em En
| MEDLINE
| ID: mdl-12515311
ABSTRACT
The effects of normothermia and delayed hypothermia on the levels of N-acetylaspartate (NAA), reduced glutathione (GSH) and the activities of mitochondrial complex I, II-III, IV and citrate synthase were measured in brain homogenates obtained from anaesthetized neonatal pigs following transient in vivo hypoxia-ischaemia. In the normothermic animals there was a significant decrease in complex I activity and in the levels of GSH and NAA when compared to the controls. Delayed hypothermia preserved NAA and GSH at control levels and enhanced the rate of complex II-III activity. There was correlation (R = 0.79) between GSH and NAA levels when data from all three experimental groups were analyzed. Citrate synthase activity was not significantly different in the three groups, indicating maintenance of mitochondrial integrity. These data suggest that delayed hypothermia affords protection of integrated mitochondrial function in the neonatal brain following transient hypoxia-ischaemia.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Córtex Cerebral
/
Ácido Aspártico
/
Hipóxia-Isquemia Encefálica
/
Glutationa
/
Hipotermia
/
Animais Recém-Nascidos
Limite:
Animals
Idioma:
En
Revista:
Neurochem Res
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Reino Unido