A computational and experimental study of the bending induced at a double-triple helix junction.
Biophys Chem
; 45(2): 143-52, 1992 Dec.
Article
em En
| MEDLINE
| ID: mdl-1286149
ABSTRACT
We have studied the conformation of a 17 base-pair homopyrimidine.homopurine triple helix formed on a fragment of duplex DNA derived from Simian Virus SV40. Gel retardation assays indicate that an 80 base-pair fragment has an altered conformation when the triple helix is formed, which is most likely to result from an induced bend in the DNA. Investigation of the detailed conformation of the double helix-triple helix junctions has been performed by means of molecular modelling. Bending on the 5' and 3' sides of the third strand oligonucleotide are not located at equivalent positions with respect to the junctions, which is explained in terms of base stacking. The junction effects on DNA structure, induced by the requirement for cytosine protonation in the Hoogsteen-bonded strand to form CGC+ base triplets, are also discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Modelos Químicos
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Biophys Chem
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
França