[Study of the clonal characteristics of autoantibodies in chronic idiopathic thrombocytopenic purpura].
Zhonghua Nei Ke Za Zhi
; 43(2): 87-9, 2004 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-15059401
ABSTRACT
OBJECTIVE:
To define the clonality characteristics of autoantibodies in chronic idiopathic thrombocytopenic purpura (ITP).METHODS:
The heavy-chain and light-chain phenotype composition of the glycoprotein (GP)-specific IgG antibodies were analysed with a modified monoclonal antibody specific immobilization of platelet antigens (MAIPA) technique. The immunoglobulin heavy-chain gene rearrangement was analysed by PCR amplification.RESULTS:
16 out of the 43 patient sera reacted with least one of the following five GPs, namely GPIIb/IIIa, GPIb, GPIa, GPIV and GPV. Eight of 11 (73%) GP-specific antibodies displayed a restricted heavy-chain phenotypes. 80% (16/20) of the GP-specific antibodies showed a restricted kappa or lambda light-chain phenotype. Moreover, in 6 patients the GP-specific antibodies were found to be both light-chain and heavy-chain restricte. Using PCR amplification of immunoglobulin heavy-chain genes, 3 patients displayed heavy-chain genes rearrangement.CONCLUSION:
The GP-specific autoantibodies are derived from a restricted number of B-cell clones in proportion of ITP patients.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autoanticorpos
/
Púrpura Trombocitopênica Idiopática
Limite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
Zh
Revista:
Zhonghua Nei Ke Za Zhi
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China