[HER2 gene amplification assay: is CISH an alternative to FISH?]. / Recherche de l'amplification du gène HER2: la CISH est-elle une alternative à la technique FISH?
Ann Pathol
; 23(6): 617-22, 2003 Dec.
Article
em Fr
| MEDLINE
| ID: mdl-15094603
ABSTRACT
The HER2 proto-oncogene encodes a transmembrane protein, which is considered to function as a growth factor receptor. Overexpression of this protein found by immunohistochemistry in about 20% of infiltrating breast carcinomas, has a predictive value of response to treatment by trastuzumab, an anti-HER2 humanized monoclonal antibody. Search for HER2 gene amplification is necessary to adapt the immunohistochemical technique quality and also in the cases of delicate analysis or weak overexpression. It is usually carried out by Fluorescence In Situ Hybridization (FISH). A more recent hybridization technique, named CISH because of its chromogenic revelation is an alternative method, which gives highly correlated results with FISH. We present details of this technique, which may be more familiar for the pathologists than FISH, because reading analysis is similar to that of immunohistochemical staining.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos Cromogênicos
/
Hibridização In Situ
/
Genes erbB-2
/
Técnicas de Amplificação de Ácido Nucleico
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
Fr
Revista:
Ann Pathol
Ano de publicação:
2003
Tipo de documento:
Article