Cytogenetic abnormalities and the failure of development after round spermatid injections.
Fertil Steril
; 81(5): 1283-8, 2004 May.
Article
em En
| MEDLINE
| ID: mdl-15136091
ABSTRACT
OBJECTIVE:
To assess cytologic and cytogenetic abnormalities following round spermatid injection.DESIGN:
Prospective analysis.SETTING:
In vitro fertilization centers. PATIENT(S) Fourteen couples accepted to a round spermatid injection (ROSI) and preimplantation genetic diagnosis (PGD) program after appropriate counseling. INTERVENTION(S) ROSI, PGD, with fluorescence in situ hybridization for chromosome enumeration. MAIN OUTCOME MEASURE(S) Cytologic and cytogenetic abnormalities in oocytes, zygotes, and blastomeres. RESULT(S) The fertilization rate following ROSI was 36%. Only 11 of 143 (7.7%) oocytes developed to have several blastomeres. Cytologic and cytogenetic abnormalities accounted for the vast majority of blockage at oocyte, zygote, and early mitotic division stages. Four biopsied embryos were normal. These and seven others were implanted, but no pregnancy was achieved. CONCLUSION(S) A PGD diagnosis for common aneuploidies and blastocyst stage transfer is feasible for ROSI cases. Failure with ROSI is cause primarily by chromosome abnormalities, so use of ROSI in assisted reproductive technologies should be limited.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermátides
/
Aberrações Cromossômicas
/
Desenvolvimento Embrionário e Fetal
Tipo de estudo:
Observational_studies
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Fertil Steril
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Turquia