[Expression and significance of Hsp70 and PCNA during the development of forestomach carcinoma in NIH mice].

AIM: To explore the expressions and significance of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the development of forestomach carcinoma in NIH mice induced with N-Nitrososarcosineethylester(NSEE). METHODS: 144 mice were divided into 6 groups and their stomachs were infused with NSEE. Then 24 mice were killed every two weeks and their forestomachs were used for the study of the expressions of Hsp70 and PCNA by immunohistochemical staining. RESULTS: With the progress of carcinogenesis, the expression pattern of Hsp70 was up-down-up, but the expression trend was still increasing. On day 56 and day 70 after NSEE treatment, the expression level of Hsp70 was higher than that of control (P<0.05). On day 84, the expression level of Hsp70 was markedly higher than that of control (P<0.01). The expression of PCNA increased gradually with the carcinogenesis. As compared with the control, stronger expression was found on day 42 and day 56 (P<0.05) and notably stronger expression on day 84 (P<0.01). The expression of Hsp70 was positively correlated to that of PCNA (r=0.98, P<0.01). CONCLUSION: In the development of forestomach carcinoma of NIH mice, the expressions of both Hsp70 and PCNA increased and were positively correlated with each other.