Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus.
Mol Immunol
; 42(1): 125-36, 2005 Jan.
Article
em En
| MEDLINE
| ID: mdl-15488951
ABSTRACT
The availability of monoclonal antibodies (mAbs) specific for the SARS-coronavirus (SARS-CoV) is important for the development of both diagnostic tools and treatment of infection. A molecular characterization of nine monoclonal antibodies raised in immune mice, using highly purified, inactivated SARS-CoV as the inoculating antigen, is presented in this report. These antibodies are specific for numerous viral protein targets, and six of them are able to effectively neutralize SARS-CoV in vitro, including one with a neutralizing titre of 0.075 nM. A phylogenetic analysis of the heavy and light chain sequences reveals that the mAbs share considerable homology. The majority of the heavy chains belong to a single Ig germline V-gene family, while considerably more sequence variation is evident in the light chain sequences. These analyses demonstrate that neutralization ability can be correlated with specific murine V(H)-gene alleles. For instance, one evident trend is high sequence conservation in the V(H) chains of the neutralizing mAbs, particularly in CDR-1 and CDR-2. The results suggest that optimization of murine mAbs for neutralization of SARS-CoV infection will likely be possible, and will aid in the development of diagnostic tools and passive treatments for SARS-CoV infection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Coronavírus Relacionado à Síndrome Respiratória Aguda Grave
/
Anticorpos Monoclonais
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Especificidade de Anticorpos
Limite:
Animals
Idioma:
En
Revista:
Mol Immunol
Ano de publicação:
2005
Tipo de documento:
Article