Mg2+-ATPase activity in suckling rat brain regions in galactosaemia in vitro. L-Cysteine and glutathione effects.
Toxicol In Vitro
; 19(2): 167-72, 2005 Mar.
Article
em En
| MEDLINE
| ID: mdl-15649629
ABSTRACT
UNLABELLED Mg2+-ATPase activity is implicated with Mg2+ homeostasis, maintaining high brain intracellular Mg2+ content. We determined the in vitro effects of galactose-1-phosphate (Gal-1-P), galactitol (Galtol) and galactose (Gal) {mix A=Gal-1-P(2 mM)+Galtol(2 mM)+Gal(4 mM) concentrations commonly found in patients with classical galactosaemia} or Galtol and Gal {mix B=Galtol(2 mM)+Gal(1 mM) concentrations usually measured in patients with galactokinase deficiency galactosaemia} on Mg2+-ATPase activity in suckling rat brain frontal cortex, hippocampus or hypothalamus homogenates. Gal-1-P significantly (p<0.001) enhanced enzyme activity in all the brain areas measured, whereas Galtol and Gal failed to cause any effect in the same regions. Mix A remarkably (p<0.001) stimulated Mg2+-ATPase in the studied areas. On the contrary, mix B had no effect. The supplementation of antioxidant l-cysteine (Cys) or reduced Glutathione (GSH) in mix A failed to reverse to normal the activated enzyme in frontal cortex and hypothalamus, while they significantly reduced Mg2+-ATPase activation in hippocampus. CONCLUSIONS:
(a) Gal-1-P enormously activated Mg2+-ATPase in all the studied brain regions, (b) Mix A, also, excessively activated the enzyme in the same areas, (c) the production of free radicals may be implicated with the enzyme activation and (d) Cys or GSH significantly decreased the activated hippocampal Mg2+-ATPase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
ATPase de Ca(2/) e Mg(2/)
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Cisteína
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Glutationa
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Antioxidantes
Limite:
Animals
Idioma:
En
Revista:
Toxicol In Vitro
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Grécia