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Mouse testican-2. Expression, glycosylation, and effects on neurite outgrowth.
Schnepp, Anke; Komp Lindgren, Patricia; Hülsmann, Hanni; Kröger, Stephan; Paulsson, Mats; Hartmann, Ursula.
Afiliação
  • Schnepp A; Center for Biochemistry, Medical Faculty, University of Cologne, D-50931 Cologne, Germany.
J Biol Chem ; 280(12): 11274-80, 2005 Mar 25.
Article em En | MEDLINE | ID: mdl-15657052
Mouse testican-2 was cloned, sequenced, and shown to be a proteoglycan with a multidomain structure closely similar to that of the human ortholog, previously described as a calcium binding extracellular matrix molecule of the BM-40/SPARC/osteonectin family (Vannahme, C., Schübel, S., Herud, M., Gösling, S., Hülsmann, H., Paulsson, M., Hartmann, U., and Maurer, P. (1999). J. Neurochem. 73, 12-20). Recombinant mouse testican-2 was used to prepare specific antibodies that allowed the detection of testican-2 in various brain structures but also in lung, testis, and in several endocrine glands. Although the testican-2 expressed in EBNA-293 cells carried both heparan sulfate and chondroitin/dermatan sulfate glycosaminoglycan chains, the tissue form always contained only heparan sulfate. Both tissue-derived and recombinant testican-2 carried N-linked glycans. Tissue-derived forms of testican-2 were detected as proteoglycans of varying size, whereas a portion of the molecules produced by EBNA-293 cells were core proteins, lacking glycosaminoglycans. Both the proteoglycan and core protein forms of testican-2 inhibited neurite extension from cultured primary cerebellar neurons and may play regulatory roles in the development of the central nervous system.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteoglicanas / Neuritos Limite: Animals Idioma: En Revista: J Biol Chem Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Alemanha
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteoglicanas / Neuritos Limite: Animals Idioma: En Revista: J Biol Chem Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Alemanha