Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly.
Bioelectrochemistry
; 66(1-2): 139-43, 2005 Apr.
Article
em En
| MEDLINE
| ID: mdl-15833714
ABSTRACT
The electrochemiluminescence (ECL) of a luminol derivate (ABEI) generated both by a carbon electrode and a polypyrrole-coated carbon electrode was examined. It was found that the polypyrrole film (ppy) did not inhibit the ECL. After that, ABEI anchored on a single stranded DNA target (ODNt) has been used for the ECL detection of the hybridization between a complementary single stranded DNA probe (ODNp) covalently linked to a polypyrrole support and the ODNt. The ECL detection has been performed using a DNA sensor having a low surface concentration of ODNp probes, constituted of a polypyrrole copolymer electrosynthesized from a pyrrole-ODNp/pyrrole monomer ratio of 1/20,000.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise de Sequência com Séries de Oligonucleotídeos
/
Luminol
/
Hibridização de Ácido Nucleico
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Bioelectrochemistry
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
França