[The effects of resveratrol on the activation, proliferation and cytokine expression of murine T lymphocytes].

ABSTRACT

AIM: To study the effects of resveratrol (RSV) on the activation, proliferation and cytokine expression of murine T lymphocytes. METHODS: Murine T lymphocytes were isolated from lymph nodes and cultured in vitro. The lymphocytes were pre-treated with RSV for 1 h prior to activation with PDB plus ionomycin. (3)H-TdR incorporation was used to detect T cell proliferation. IL-2 and IFN-gamma mRNA were detected by RT-PCR, IL-2 and IFN-gamma proteins were detected by intracellular cytokine staining with flow cytometry. RESULTS: RSV can inhibit the proliferation and expression of IL-2 and IFN-gamma of T lymphocytes. CONCLUSION: The immunosuppressive action of RSV may be related with its inhibitory effects on T cell activation, proliferation and cytokine expression.