In vivo clearance of hepatitis C virus nonstructural 3/4A-expressing hepatocytes by DNA vaccine-primed cytotoxic T lymphocytes.
J Infect Dis
; 192(12): 2112-6, 2005 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-16288375
ABSTRACT
A key question in the development of a therapeutic vaccine against hepatitis C virus (HCV) is whether vaccine-primed T cells enter the liver and eliminate HCV-expressing hepatocytes. In the absence of an infectious small-animal model, we evaluated liver homing of vaccine-primed T cells in mice with transient hepatic transgene expression of the HCV nonstructural 3/4A (NS3/4A) protein. We found that T cells primed by transdermal DNA-based vaccination entered the liver and cleared NS3/4A-expressing hepatocytes in transiently transgenic CD8(+/+) mice but not in CD8(-/-) mice. Hence, peripherally primed NS3/4A-specific CD8(+) T cells home to the liver and clear HCV protein-expressing hepatocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vacinas contra Hepatite Viral
/
Linfócitos T Citotóxicos
/
Hepatite C
/
Proteínas não Estruturais Virais
/
Vacinas de DNA
/
Hepatócitos
Limite:
Animals
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Suécia