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The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8.
Hou, Haitong; Subramanian, Kanagaraj; LaGrassa, Tracy J; Markgraf, Daniel; Dietrich, Lars E P; Urban, Jörg; Decker, Nadine; Ungermann, Christian.
Afiliação
  • Hou H; Biochemie-Zentrum der Universität Heidelberg, Germany.
Proc Natl Acad Sci U S A ; 102(48): 17366-71, 2005 Nov 29.
Article em En | MEDLINE | ID: mdl-16301533
Vacuole biogenesis depends on specific targeting and retention of peripheral membrane proteins. At least three palmitoylated proteins are found exclusively on yeast vacuoles: the fusion factor Vac8, the kinase Yck3, and a novel adaptor protein implicated in microautophagy, Meh1. Here, we analyze the role that putative acyltransferases of the DHHC family play in their localization and function. We find that Pfa3/Ynl326c is required for efficient localization of Vac8 to vacuoles in vivo, while Yck3 or Meh1 localization is not impaired in any of the seven DHHC deletions. Vacuole-associated Vac8 appears to be palmitoylated in a pfa3 mutant, but this population is refractive to further palmitoylation on isolated vacuoles. Vacuole morphology and inheritance, which both depend on Vac8 palmitoylation, appear normal, although there is a reduction in vacuole fusion. Interestingly, Pfa3 is required for the vacuolar localization of not only an SH4 domain that is targeted by myristate/palmitate (as in Vac8) but also one that is targeted by a myristate/basic stretch (as in Src). Our data indicate that Pfa3 has an important but not exclusive function for Vac8 localization to the vacuole.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Palmitoil Coenzima A / Saccharomyces cerevisiae / Vacúolos / Aciltransferases / Proteínas de Saccharomyces cerevisiae / Lipoproteínas / Proteínas de Membrana Tipo de estudo: Risk_factors_studies Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Palmitoil Coenzima A / Saccharomyces cerevisiae / Vacúolos / Aciltransferases / Proteínas de Saccharomyces cerevisiae / Lipoproteínas / Proteínas de Membrana Tipo de estudo: Risk_factors_studies Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2005 Tipo de documento: Article País de afiliação: Alemanha