Downregulation of Thy1 in retinal ganglion cells in experimental glaucoma.
Curr Eye Res
; 31(3): 265-71, 2006 Mar.
Article
em En
| MEDLINE
| ID: mdl-16531284
ABSTRACT
PURPOSE:
Thy1 is a surface glycoprotein uniquely expressed in retinal ganglion cells (RGCs) in retina. The aim of this study was to investigate the expression change of Thy1 in a model of experimental glaucoma.METHODS:
The change of protein and mRNA levels of Thy1 in the retina were studied using stereological counts of back-labeled RGCs, Western blot analysis, immunohistochemistry, and laser capture microdissection (LCM) of RGCs with quantitative PCR analysis of mRNA in a model of experimental glaucoma. LCM after optic nerve crush was also performed to evaluate Thy1 mRNA levels after a different injury.RESULTS:
After 10 days of elevated IOP, there was a 34% loss of RGC number, Thy1 protein decreased 60% in eyes with elevated intraocular pressure (IOP), and Thy1 mRNA levels decreased 51% in RGCs. Both protein and mRNA level change of Thy1 is to a much greater extent than RGC number loss.CONCLUSIONS:
The current results confirm that Thy 1 mRNA levels do not reflect the number of RGCs present and extend this to include a parallel decrease in Thy1 protein levels. These results suggest that Thy1 serves as an early marker of RGC stress, but not a marker of RGC loss, in models of retinal damage.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Ganglionares da Retina
/
RNA Mensageiro
/
Glaucoma
/
Antígenos Thy-1
Limite:
Animals
Idioma:
En
Revista:
Curr Eye Res
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos