Inactivation of infectious bursal disease virus by binary ethylenimine and formalin.
J Zhejiang Univ Sci B
; 7(4): 320-3, 2006 Apr.
Article
em En
| MEDLINE
| ID: mdl-16532536
ABSTRACT
In this experiment conducted to study the inactivation dynamics of infectious bursal disease virus (IBDV) by binary ethylenimine (BEI) in comparison with formalin, IBDV was isolated from the bursa of infected chickens and its confirmation was done by agar gel precipitation test. Viral suspensions were subjected to inactivation with BEI and formalin for pre-set time intervals. BEI was employed at concentrations of 0.001 and 0.002 mol/L while formalin was used at 0.1% and 0.2%. Sampling was done at 6, 12, 24, 36 and 48 h of incubation and samples were tested for their inactivation status in 9-day-old embryonated eggs and 3-week-old broiler chickens. IBDV was completely inactivated by 0.001 and 0.002 mol/L BEI after 36 h of incubation at 37 degrees C, whereas formalin at 0.1% and 0.2% concentrations inactivated IBDV in 24 h.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Aziridinas
/
Vacinas Virais
/
Galinhas
/
Infecções por Birnaviridae
/
Vírus da Doença Infecciosa da Bursa
/
Formaldeído
Limite:
Animals
Idioma:
En
Revista:
J Zhejiang Univ Sci B
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Paquistão