Role of quercetin and its in vivo metabolites in protecting H9c2 cells against oxidative stress.
Biochimie
; 89(1): 73-82, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17045724
ABSTRACT
The aim of this study was to investigate the potential of quercetin and two of its "in vivo" metabolites, 3'-O-methyl quercetin and 4'-O-methyl quercetin, to protect H9c2 cardiomyoblasts against H(2)O(2)-induced oxidative stress. As limited data are available regarding the potential uptake and cellular effects of quercetin and its metabolites in cardiac cells, we have evaluated the cellular association/uptake of the three compounds and their involvement in the modulation of two pro-survival signalling pathways ERK1/2 signalling cascade and PI3K/Akt pathway. The three flavonols associated with cells to differing extents. Quercetin and its two O-methylated metabolites were able to reduce intracellular ROS production but only quercetin was able to counteract H(2)O(2) cell damage, as measured by MTT reduction assay, caspase-3 activity and DNA fragmentation assays. Furthermore, only quercetin was observed to modulate pro-survival signalling through ERK1/2 and PI3K/Akt pathway. In conclusion we have demonstrated that quercetin, but not its O-methylated metabolites, exerts protective effects against H(2)O(2) cardiotoxicity and that the mechanism of its action involves the modulation of PI3K/Akt and ERK1/2 signalling pathways.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quercetina
/
Transdução de Sinais
/
Estresse Oxidativo
/
Antioxidantes
Limite:
Animals
Idioma:
En
Revista:
Biochimie
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Itália