Molecular typing of a Legionella pneumophila outbreak in Ontario, Canada.
J Med Microbiol
; 56(Pt 3): 336-341, 2007 Mar.
Article
em En
| MEDLINE
| ID: mdl-17314363
ABSTRACT
An outbreak of Legionnaires' disease at a long-term care facility in Ontario, Canada from September to October 2005 resulted in the death of 23 residents and the illness of 112 other people. In response, molecular methods were developed to detect Legionella pneumophila in clinical lung samples and to subtype isolates from clinical and environmental samples. The targeted genetic loci included Legionella-specific virulence determinants (mip, icmO, sidA and lidA) and core bacterial determinants (ftsZ, trpS and dnaX). An established amplified fragment length polymorphism typing method provided the first indication of genetic relatedness between strains recovered from clinical samples and strains cultured from environmental samples taken from the outbreak site. These associations were verified using the European Working Group for Legionella Infections sequence-based typing protocol targeting the flaA, pilE, asd, mip, mompS and proA loci. These molecular typing methods confirmed the outbreak source as a contaminated air conditioning cooling tower.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Legionelose
/
Surtos de Doenças
/
Técnicas de Tipagem Bacteriana
/
Legionella pneumophila
Tipo de estudo:
Guideline
Limite:
Humans
País/Região como assunto:
America do norte
Idioma:
En
Revista:
J Med Microbiol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Canadá