Tumor necrosis factor alpha induces apoptosis in mammary adenocarcinoma cells by an increase in intranuclear free Ca2+ concentration and DNA fragmentation.
Cancer Res
; 52(5): 1342-6, 1992 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-1737395
ABSTRACT
The incubation of human mammary adenocarcinoma cells (BT-20) with tumor necrosis factor alpha in the absence or presence of cycloheximide resulted in progressive DNA fragmentation. This was preceded by a sustained increase in intracellular free Ca2+ concentration and was not detected in cells pretreated with intracellular Ca2+ chelators, calmodulin antagonists, or activators of protein kinase C. Image analysis of fura-2-loaded BT-20 cells treated with tumor necrosis factor alpha revealed that, in many cells, the initial increase in Ca2+ level occurred in a cellular region that corresponded to the localization of the nucleus. Our findings suggest that tumor necrosis factor alpha can promote an increase in intranuclear free Ca2+ which, in turn, may stimulate Ca(2+)-dependent endonuclease activity, resulting in DNA fragmentation and apoptosis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
/
Neoplasias da Mama
/
DNA de Neoplasias
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Adenocarcinoma
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Cálcio
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Fator de Necrose Tumoral alfa
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Morte Celular
Limite:
Humans
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Itália