Laser-induced photofragmentation of neutral and acidic glycans inside an ion-trap mass spectrometer.
Rapid Commun Mass Spectrom
; 21(8): 1452-60, 2007.
Article
em En
| MEDLINE
| ID: mdl-17385789
ABSTRACT
Permethylated acidic and neutral N-glycans representing different types of glycan structures, such as linear and branched sialylated structures, high-mannose type and fucosylated complex type, were photodissociated with 157 nm vacuum ultraviolet light in a linear ion trap. Cross-ring fragments corresponding to high-energy fragmentation pathways were observed in abundance for all studied structures. Some product ions appear diagnostic for a linkage of sialic acid residues and the glycan antenna to which these residues are attached. A conclusive assignment of the fucosylation site of the studied glycan structure has been facilitated through measurement of cross-ring fragmentation resulting from photodissociation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Espectrometria de Massas por Ionização por Electrospray
/
Lasers
Limite:
Humans
Idioma:
En
Revista:
Rapid Commun Mass Spectrom
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos