Analysis of the inhibition of the ergosterol pathway in fungi using the atmospheric solids analysis probe (ASAP) method.

ABSTRACT

Fungal cells treated with various inhibitors to the ergosterol pathway were analyzed using an Orbitrap mass spectrometer equipped with an atmospheric solids analysis probe (ASAP). The technique is a rapid means for determining which of the multiple steps in the ergosterol pathway were interrupted by an inhibitor. Furthermore, in an inhibitor concentration study, the ASAP method was able to rapidly provide an estimate of the effectiveness of inhibition. In this method the cells are inserted directly into a hot nitrogen stream, thus eliminating extensive sample workup before analysis. Data indicating the point of pathway interruption are obtained in seconds.