Identification of 12(S)-hydroxyeicosatetraenoic acid in the young rat lens.
Curr Eye Res
; 10(4): 331-7, 1991 Apr.
Article
em En
| MEDLINE
| ID: mdl-1906393
ABSTRACT
Evidence is presented indicating that young rat lens has the capacity to synthesize 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE) from exogenous arachidonic acid (AA). A 9,000 xg supernatant prepared from 15 day old rat lenses, when incubated with calcium and U-[14C]-AA, generated a radiolabelled product with a retention time identical to authentic unlabelled 12-HETE in two different HPLC solvent systems. Mass spectral analysis provided evidence that the metabolite was 12-HETE while chiral studies demonstrated the exclusive presence of the 12(S) isomer. Radiolabelled 12-HETE synthesis was inhibited by preincubating the 15 day old rat lens supernatant with 0.2 Mm curcumin, a mixed cyclooxygenase/lipoxygenase inhibitor. Radiolabelled 12(S)-HETE synthetic capacity was highest in the 4 day old rat lens, the earliest time period measured, and rapidly declined with age reaching negligible levels at about 4 months. These results suggest that the young rat lens possess the biosynthetic capacity to generate radiolabelled 12(S)-HETE from U-[14]C-AA. The profile of 12-HETE synthetic activity suggests a possible regulatory function of the hydroxylated AA metabolite in the developing lens.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Hidroxieicosatetraenoicos
/
Cristalino
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Curr Eye Res
Ano de publicação:
1991
Tipo de documento:
Article