In vitro metabolism of flax lignans by ruminal and faecal microbiota of dairy cows.
J Appl Microbiol
; 105(5): 1585-94, 2008 Nov.
Article
em En
| MEDLINE
| ID: mdl-19146494
ABSTRACT
AIMS:
To determine the in vitro conversion of plant lignans from two flax products (hull and seed) into the mammalian lignans, enterolactone and enterodiol, by bovine ruminal and faecal microbiota. METHODS ANDRESULTS:
Flax seeds and hulls were incubated in vitro over a 96-h time course with ruminal or faecal inoculum. Plant lignans in flax seeds and hulls averaged 9.2 and 32.0 nmol mg(-1), respectively. The highest net production of enterodiol at 72 and 96 h of incubation was obtained with flax hulls incubated with faecal microbiota. There was no difference in net production of enterodiol between flax products within the first 24 h of incubation. In general, net production of enterolactone over the 96-h time course was significantly higher for flax products incubated with ruminal than with faecal microbiota. Net production of enterolactone at 72 and 96 h of incubation was greater for flax hulls than flax seeds.CONCLUSIONS:
Results of the present experiment suggest that, of the metabolites studied, the main mammalian lignan metabolite produced from flax hulls and seeds by ruminal microbiota is enterolactone while faecal microbiota leads mainly to the net production of enterodiol. SIGNIFICANCE AND IMPACT OF THE STUDY This research will improve the understanding of the metabolic pathway of mammalian lignans in dairy cows, in order to enable targeted manipulation of their quantities in milk.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rúmen
/
4-Butirolactona
/
Lignanas
/
Linho
/
Fezes
Limite:
Animals
Idioma:
En
Revista:
J Appl Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Canadá