Novel mutations of the FASN gene and their effect on fatty acid composition in Japanese Black beef.
Biochem Genet
; 47(5-6): 397-411, 2009 Jun.
Article
em En
| MEDLINE
| ID: mdl-19291389
ABSTRACT
Eight novel and four known mutations were detected in the coding sequence of the bovine fatty acid synthase (FASN) gene of an F2 population from Japanese Black and Limousin cattle. Two mutations, g.16024A>G and g.16039T>C, detected in exon 34, which determine amino acid substitutions of threonine (T) to alanine (A) and tryptophan (W) to arginine (R), were clearly separated in the parental breeds. The haplotypes (TW and AR) segregated in F2 individuals and had a significant effect on the fatty acid composition of backfat, intermuscular fat, and intramuscular fat. The TW haplotype was associated with increasing C180 and C181 content and the ratio of monounsaturated to saturated fatty acids, and decreasing C140, C141, C160, and C161 content. The two mutations were screened in two commercial Japanese Black half-sibling populations and similarly determined the contribution to the fatty acid composition of intramuscular fat. The frequency of the TW haplotype was markedly higher in Japanese Black (0.67) than in Holstein (0.17), Angus (0.02), and Hereford (0.07). We conclude that these mutations may contribute to the characteristic fatty acid composition of Japanese Black beef.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bovinos
/
Ácido Graxo Sintases
/
Ácidos Graxos
/
Carne
/
Mutação
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Biochem Genet
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Japão