Simultaneous electrochemical monitoring of metabolites related to the xanthine oxidase pathway using a grinded carbon electrode.
Anal Chem
; 81(11): 4302-7, 2009 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-19402672
ABSTRACT
Using a mechanically grinded pyrolytic graphite electrode in edge orientation, a sensitive electrochemical method was developed for simultaneous determination of uric acid (UA), xanthine (XAN), hypoxanthine (HYP) (products of purine catabolism in human), allopurinol (ALO), and oxypurinol (OXY) (a drug used in treatment of purine catabolism disorders and its metabolite, respectively). It is demonstrated that differential pulse voltammetry in connection with this electrode can serve as a simple and efficient tool for monitoring transformation of purine catabolites (HYP --> XAN --> UA) catalyzed by xanthine oxidase (XO) as well as inhibition of this pathway by ALO being enzymatically converted to OXY. Our protocol is based on direct electrochemical measurement of oxidation peaks for each of the substances during in vitro reactions in a single detection step by the same electrode system. In addition, we show that the proposed electrochemical technique can be applied to parallel detection of metabolites involved in the XO pathway excreted in urine without any pretreatment of the clinical samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxipurinol
/
Purinonas
/
Xantina Oxidase
/
Alopurinol
/
Técnicas Eletroquímicas
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Guideline
Limite:
Humans
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
República Tcheca