Interference of Dermatophagoides' specific immunoglobulins G in the quantification of mite's specific immunoglobulins E.
J Immunoassay Immunochem
; 30(3): 338-47, 2009.
Article
em En
| MEDLINE
| ID: mdl-19591046
ABSTRACT
In order to investigate the interference of specific IgG in the quantification of specific IgE, using the ImmunoCAP 250 system, we studied, in parallel, the interference by total adsorption of interferent and the classical method of adding interferent increasingly. Furthermore, to evaluate if the interference is affected by different solid phases, total extract of Dermatophagoides pteronyssinus and recombinant allergens of Dermatophagoides farinae were used. The results showed a statistical significant interference by IgG in the quantification of the specific IgE, but neither analytical nor clinical significant interference were observed. Therefore, this analytical system provides an accurate method for determination of the specific IgE concentration contributing to the allergic disease diagnosis quality.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina E
/
Imunoglobulina G
/
Dermatophagoides farinae
/
Dermatophagoides pteronyssinus
/
Erros de Diagnóstico
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
/
Adult
/
Animals
/
Child
/
Child, preschool
/
Humans
/
Middle aged
Idioma:
En
Revista:
J Immunoassay Immunochem
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Portugal