Ultrafast dynamics of protein proton transfer on short hydrogen bond potential energy surfaces: S65T/H148D GFP.
J Am Chem Soc
; 132(5): 1452-3, 2010 Feb 10.
Article
em En
| MEDLINE
| ID: mdl-19916498
ABSTRACT
Ultrafast proton transfer dynamics on a short H-bond in a protein were studied through the time-resolved fluorescence of the S65T/H148D green fluorescent protein (GFP) mutant. In response to the change in chromophore pK(a) upon excitation, the donor-proton-acceptor structure evolves on a sub-100 fs time scale, followed by picosecond time scale vibrational cooling and host structure reorganization.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prótons
/
Proteínas de Fluorescência Verde
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Reino Unido