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Donor apoptotic lymphocyte transfusion-induced liver allograft tolerance by up-regulation of CD4(+)CD25(+) regulatory T cells in peripheral blood.
Cheng, J; Zhou, L; Qin, Y S; Wang, Y; Xie, H Y; Feng, X W; Zheng, S S.
Afiliação
  • Cheng J; Department of Surgery, the First Affiliated Hospital, Zhejiang University, Zhejiang Province, People's Republic of China.
Transplant Proc ; 41(9): 3893-7, 2009 Nov.
Article em En | MEDLINE | ID: mdl-19917407
Uptake of apoptotic cells by antigen-presenting cells (APC) may be involved in tolerance maintenance with an immunoregulatory role. The aim of this study was to evaluate the consequences of preoperative transfusion of donor apoptotic lymphocytes on survival of orthotopic liver transplantations (OLT). OLT was performed between Lewis (donor) and Brown Norway (BN recipient) inbred rats using a double-cuff technique. Apoptotic splenic lymphocytes induced by ultraviolet-C (UVC) irradiation were infused intravenously at 7 days before OLT. Changes in regulatory T cells in blood were determined using flow cytometry. UVC irradiated lymphocytes were sensitive and effective, as evidenced by increased peripheral blood CD4(+)CD25(+) T cells compared with recipients that received infusion of untreated donor lymphocytes or a control. Apoptotic lymphocyte transfusion prolonged hepatic allograft survival, with significantly lower histological stages of inflammation and cellular infiltration than in untreated allografts. Our results demonstrated that donor apoptotic cells promoted allograft acceptance and up-regulated CD4(+)CD25(+) regulatory T cells (Treg) in blood.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos / Transplante de Fígado / Linfócitos T Reguladores / Tolerância ao Transplante Limite: Animals Idioma: En Revista: Transplant Proc Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos / Transplante de Fígado / Linfócitos T Reguladores / Tolerância ao Transplante Limite: Animals Idioma: En Revista: Transplant Proc Ano de publicação: 2009 Tipo de documento: Article