Ca2+ is essential for multistep activation of the heat shock factor in permeabilized cells.
Mol Cell Biol
; 11(6): 3365-8, 1991 Jun.
Article
em En
| MEDLINE
| ID: mdl-2038338
ABSTRACT
We have developed a novel permeabilized-cell system to study transcription mechanisms. In permeabilized cells, heat-induced activation of the heat shock factor and transcription of the hsp70 gene require Ca2+. Activation involves at least two steps:
Ca(2+)- and heat-dependent activation of heat shock factor binding and a second step, prior to transcription of hsp70, that requires ATP and is sensitive to genistein, a protein kinase inhibitor.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cálcio
/
Proteínas de Ligação a DNA
/
Proteínas de Choque Térmico
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1991
Tipo de documento:
Article