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Elastin-like recombinamers as substrates for retinal pigment epithelial cell growth.
Srivastava, Girish K; Martín, Laura; Singh, Amar K; Fernandez-Bueno, Ivan; Gayoso, Manuel J; Garcia-Gutierrez, Maria T; Girotti, Alessandra; Alonso, Matilde; Rodríguez-Cabello, José C; Pastor, José C.
Afiliação
  • Srivastava GK; Institute of Applied Ophthalmobiology (IOBA), University of Valladolid, Valladolid, Spain. girish@ioba.med.uva.es
J Biomed Mater Res A ; 97(3): 243-50, 2011 Jun 01.
Article em En | MEDLINE | ID: mdl-21442725
The aim of this study is to investigate the use of elastin-like recombinamers (ELRs) as a substrate that can maintain the growth, phenotype, and functional characteristics of retinal pigment epithelial (RPE) cells efficiently and as a suitable carrier for the transplantation of autologous RPE cells for treatment of age-related macular degeneration (AMD). ELR films containing a bioactive sequence, RGD (ELR-RGD), and one with no specific sequence (ELR-IK) as control, were obtained by solvent-casting onto glass and subsequent cross-linking. ARPE19 cells were seeded on sterilized ELR films as well as on the control surfaces. Cells were analysed after 4, 24, 72, and 120 h to study cell adhesion, proliferation, cell viability, morphology, and specificity by staining with Trypan blue, DAPI, Rhodamin-Phalloidin and RPE65, ZO-1 antibodies and observing under fluorescence as well as electron microscope. ARPE19 cells seeded on both ELR films and controls were 100% viable and maintained their morphology and set of characteristics at the different time points studied. Cell proliferation on ELR-RGD was significantly higher than that found on ELR-IK at all time points, although it was less than the growth rate on polystyrene. ARPE19 cells grow well on ELR-RGD maintaining their phenotype. These results should be extended to further studies with fresh human RPE cells and in vivo studies to determine whether this ELR-RGD matrix could be used as a Bruch's membrane prosthesis and carrier for transplantation of RPE cells in patients suffering with AMD.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Regeneração / Elastina / Proliferação de Células / Epitélio Pigmentado da Retina / Degeneração Macular Limite: Humans Idioma: En Revista: J Biomed Mater Res A Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Espanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Regeneração / Elastina / Proliferação de Células / Epitélio Pigmentado da Retina / Degeneração Macular Limite: Humans Idioma: En Revista: J Biomed Mater Res A Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Espanha