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Novel disposable biochip platform employing supercritical angle fluorescence for enhanced fluorescence collection.
Hill, Duncan; McDonnell, Barry; Hearty, Stephen; Basabe-Desmonts, Lourdes; Blue, Robert; Trnavsky, Michal; McAtamney, Colm; O'Kennedy, Richard; MacCraith, Brian D.
Afiliação
  • Hill D; Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland.
Biomed Microdevices ; 13(4): 759-67, 2011 Aug.
Article em En | MEDLINE | ID: mdl-21559870
ABSTRACT
This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a planar substrate. The optical elements are based on supercritical angle fluorescence (SAF) which provides substantial enhancement of the fluorescence collection efficiency but also confines the fluorescence detection volume strictly to the immediate proximity of the biochip surface, thereby having the potential to discriminate against background fluorescence from the analyte solution. An optical reader is also described that enables interrogation and fluorescence collection from the nine optical elements on the chip. The sensitivity of the system was determined with a biotin-avidin assay while its clinical utility was demonstrated in an assay for C-reactive protein (CRP), an inflammation marker.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise Serial de Proteínas Limite: Humans Idioma: En Revista: Biomed Microdevices Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Irlanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise Serial de Proteínas Limite: Humans Idioma: En Revista: Biomed Microdevices Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Irlanda