Development of a method to produce hemoglobin in a bioreactor culture of Escherichia coli BL21(DE3) transformed with a plasmid containing Plesiomonas shigelloides heme transport genes and modified human hemoglobin genes.
Appl Environ Microbiol
; 77(18): 6703-5, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21803893
ABSTRACT
We describe a method for production of recombinant human hemoglobin by Escherichia coli grown in a bioreactor. E. coli BL21(DE3) transformed with a plasmid containing hemoglobin genes and Plesiomonas shigelloides heme transport genes reached a cell dry weight of 83.64 g/liter and produced 11.92 g/liter of hemoglobin in clarified lysates.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Biotecnologia
/
Hemoglobinas
/
Plesiomonas
/
Escherichia coli
/
Vias Biossintéticas
Limite:
Humans
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos