Enchanced levels of apolipoprotein M during HBV infection feedback suppresses HBV replication.
Lipids Health Dis
; 10: 154, 2011 Aug 29.
Article
em En
| MEDLINE
| ID: mdl-21875437
ABSTRACT
BACKGROUND:
Chronic liver diseases can interfere with hepatic metabolism of lipoproteins, apolipoproteins. Hepatitis B virus (HBV) is a major etiological agent causing acute and chronic liver diseases. Apolipoprotein M (ApoM) is a high-density lipoprotein (HDL) apolipoprotein and exclusively expressed in the liver parenchyma cells and in the tubular cells of the kidney. This study was to determine the correlation between HBV infection and ApoM expression. MATERIALS ANDMETHODS:
Serum ApoM levels in patients with HBV infection and in healthy individuals were measured by ELISA, ApoM mRNA expression were determined by RT-PCR, and the expression of S and E proteins of HBV, as well as the synthesis of viral DNA were measured by ELISA and real-time PCR.RESULTS:
The levels of serum ApoM was significantly elevated in patients as compared to healthy individuals (P < 0.001), ApoM promoter activity, mRNA and protein expression were all stimulated in cells transfected with infectious HBV clone. In addition, ApoM decreases the expression of S and E proteins of HBV and the synthesis of viral DNA.CONCLUSION:
Raised ApoM levels in HBV infection may in turn suppress HBV replication, one of the protective mechanisms of nature.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apolipoproteínas
/
Replicação Viral
/
Vírus da Hepatite B
/
Hepatite B Crônica
/
Lipocalinas
Tipo de estudo:
Observational_studies
Limite:
Humans
Idioma:
En
Revista:
Lipids Health Dis
Assunto da revista:
BIOQUIMICA
/
METABOLISMO
Ano de publicação:
2011
Tipo de documento:
Article