Current perspectives and recommendations for the development of mass spectrometry methods for the determination of allergens in foods.
J AOAC Int
; 94(4): 1026-33, 2011.
Article
em En
| MEDLINE
| ID: mdl-21919335
ABSTRACT
Allergen detection and quantification is an essential part of allergen management as practiced by food manufacturers. Recently, protein MS methods (in particular, multiple reaction monitoring experiments) have begun to be adopted by the allergen detection community to provide an alternative technique to ELISA and PCR methods. MS analysis of proteins in foods provides additional challenges to the analyst, both in terms of experimental design and methodology:
(1) choice of analyte, including multiplexing to simultaneously detect several biologically relevant molecules able to trigger allergic reactions; (2) choice of processing stable peptide markers for different target analytes that should be placed in publicly available databases; (3) markers allowing quantification (e.g., through standard addition or isotopically labeled peptide standards); (4) optimization of protease digestion protocols to ensure reproducible and robust method development; and (5) effective validation of methods and harmonization of results through the use of naturally incurred reference materials spanning several types of food matrix.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Alérgenos
/
Análise de Alimentos
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
J AOAC Int
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Reino Unido