Integration of monoclonal antibodies in quantitative immunoelectrophoresis by indirect immunoprecipitation.
J Immunol Methods
; 132(1): 127-35, 1990 Aug 28.
Article
em En
| MEDLINE
| ID: mdl-2202762
ABSTRACT
Characterization of the specificity of monoclonal antibody in crossed immunoelectrophoresis has been achieved by mixing the monoclonal antibody with mycobacterial antigen in the circular antigen well. After electrophoresis in the first dimension, the separated antigens and antigen complexed with monoclonal antibody were run into an intermediate gel containing rabbit anti-mouse immunoglobulin and a top gel with polyvalent rabbit anti-BCG immunoglobulin. Monoclonal antibody with bound antigen precipitated in the intermediate gel while the other antigens precipitated in their reference positions. The method was simple, efficient and sensitive. Selected monoclonal antibodies were used to demonstrate the characteristic features of the method. In rocket immunoelectrophoresis the rocket height of a monoclonal antibody may be significantly altered by adding the relevant antigen. This principle can be exploited when the polyvalent antibodies do not precipitate the antigen, and it may be used for efficient screening of monoclonal hybridoma culture supernatants. This approach may permit the quantitation of an antigen when only monoclonal antibody is available.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoeletroforese Bidimensional
/
Imunoeletroforese
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Noruega