Control of solid tumor growth in mice using EGF receptor-targeted RNA replicase-based plasmid DNA.
Nanomedicine (Lond)
; 7(4): 475-91, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22296186
ABSTRACT
AIM:
Previously, it was shown that treatment of tumor-bearing mice with an RNA replicase-based plasmid that produces dsRNA when transfected into tumor cells significantly inhibited the tumor growth. In the present study, the feasibility of further improving the anti-tumor activity of the RNA replicase-based plasmid by targeting it into tumors cells was evaluated. MATERIAL &METHODS:
An EGF-conjugated, polyethylene glycosylated cationic liposome was developed to deliver the RNA replicase-based plasmid, pSIN-ß, into EGF receptor-overexpressing human breast cancer cells (MDA-MB-468) in vitro and in vivo.RESULTS:
Delivery of pSIN-ß using the EGF receptor-targeted liposome more effectively controlled the growth of MDA-MB-468 tumors (and human epidermoid carcinoma A431 tumors) in mice than using untargeted liposome. The pSIN-ß carried by the EGF receptor-targeted liposome caused the complete regression of MDA-MB-468 tumors in mice, probably due to the enhancement of its proapoptotic, antiproliferative and antiangiogenic activities.DISCUSSION:
Tumor-targeted RNA replicase-based plasmids hold a strong potential in tumor therapy.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Neoplasias da Mama
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RNA Polimerase Dependente de RNA
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DNA
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Receptores ErbB
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Nanomedicine (Lond)
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos