Identification of hepatitis C virus transmission using a next-generation sequencing approach.
J Clin Microbiol
; 50(4): 1461-3, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22301026
ABSTRACT
Here, we describe a transmission event of hepatitis C virus (HCV) among injection drug users. Next-generation sequencing (NGS) was used to assess the intrahost viral genetic variation. Deep amplicon sequencing of HCV hypervariable region 1 allowed for a detailed analysis of the structure of the viral population. Establishment of the genetic relatedness between cases was accomplished by phylogenetic analysis. NGS is a powerful tool with applications in molecular epidemiology studies and outbreak investigations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Abuso de Substâncias por Via Intravenosa
/
Hepatite C
/
Hepacivirus
/
Sequenciamento de Nucleotídeos em Larga Escala
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
Limite:
Adult
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
México