Natural rubber biosynthesis in plants: rubber transferase.
Methods Enzymol
; 515: 63-82, 2012.
Article
em En
| MEDLINE
| ID: mdl-22999170
ABSTRACT
Rubber biosynthesis in plants is a fascinating biochemical system, which evolved at the dawn of the dicotyledoneae and is present in at least four of the dictolydonous superorders. Rubber biosynthesis is catalyzed by a membrane complex in a monolayer membrane envelope, requires two distinct substrates and a divalent cation cofactor, and produces a high-molecular-weight isoprenoid polymer. A solid understanding of this system underpins valuable papers in the literature. However, the published literature is rife with unreliable reports in which the investigators have fallen into traps created by the current incomplete understanding of the biochemistry of rubber synthesis. In this chapter, we attempt to guide both new and more established researchers around these pitfalls.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Borracha
/
Transferases
/
Asteraceae
Limite:
Animals
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos