[Increase in resistance of A431 cancer cells to TRAIL-induced apoptosis in confluent cultures].
Biofizika
; 57(4): 649-54, 2012.
Article
em Ru
| MEDLINE
| ID: mdl-23035531
It was shown that cancer cells acquired resistance to TRAIL-induced apoptosis in confluent cultures. Recombinant protein izTRAIL induced apoptosis of human carcinoma A431 cells in the first hours after cell plating at a concentration of 3-10 ng/ml, while in confluent cultures these cells acquire resistance to protein izTRAIL even at the concentration of 2 mkg/ml. Detachment and suspending of the cells of confluent cultures immediately suppressed the resistance to izTRAIL. The cells of confluent cultures, being resistant to TRAIL-induced apoptosis continue progression through the cell cycle, as evidenced by the DNA cytograms and the Ki67p-GFP reporter system. Thus, the results showed that tumor A431 cells can acquire resistance to TRAIL-induced apoptosis in confluent cultures, while continue progression through the cell cycle, keeping the proliferative potential.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Apoptose
/
Técnicas de Cultura de Células
/
Ligante Indutor de Apoptose Relacionado a TNF
Limite:
Humans
Idioma:
Ru
Revista:
Biofizika
Ano de publicação:
2012
Tipo de documento:
Article