Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain.
BMC Complement Altern Med
; 12: 225, 2012 Nov 22.
Article
em En
| MEDLINE
| ID: mdl-23173601
ABSTRACT
BACKGROUND:
Orexin A (OXA, hypocretin/hcrt 1) is a newly discovered potential analgesic substance. However, whether OXA is involved in acupuncture analgesia remains unknown. The present study was designed to investigate the involvement of spinal OXA in electroacupuncture (EA) analgesia.METHODS:
A modified rat model of post-laparotomy pain was adopted and evaluated. Von Frey filaments were used to measure mechanical allodynia of the hind paw and abdomen. EA at 2/15 Hz or 2/100 Hz was performed once on the bilateral ST36 and SP6 for 30 min perioperatively. SB-334867, a selective orexin 1 receptor (OX1R) antagonist with a higher affinity for OXA than OXB, was intrathecally injected to observe its effect on EA analgesia.RESULTS:
OXA at 0.3 nmol and EA at 2/15 Hz produced respective analgesic effects on the model (P<0.05). Pre-surgical intrathecal administered of SB-334867 30 nmol antagonized OXA analgesia and attenuated the analgesic effect of EA (P<0.05). However, SB-334867 did not block fentanyl-induced analgesia (P>0.05). In addition, naloxone, a selective opioid receptor antagonist, failed to antagonize OXA-induced analgesia (P>0.05).CONCLUSIONS:
The results of the present study indicate the involvement of OXA in EA analgesia via OX1R in an opioid-independent way.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dor
/
Complicações Pós-Operatórias
/
Coluna Vertebral
/
Neuropeptídeos
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Eletroacupuntura
/
Receptores de Neuropeptídeos
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Receptores Acoplados a Proteínas G
/
Peptídeos e Proteínas de Sinalização Intracelular
/
Analgesia
Limite:
Animals
Idioma:
En
Revista:
BMC Complement Altern Med
Assunto da revista:
TERAPIAS COMPLEMENTARES
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China